Roles of fetal G gamma-globin promoter elements and the adult beta-globin 3' enhancer in the stage-specific expression of globin genes.
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چکیده
منابع مشابه
Quantitation of human gamma globin genes and gamma globin mRNA with purified gamma globin complementary DNA.
Complementary DNA (cDNA) specific for gamma-globin nucleotide sequences has been prepared by hybridizing total cDNA made from cord blood messenger RNA (mRNA) as template to an excess of normal adult human globin mRNA and recovering the single-stranded cDNA from hydroxylapatite. The specificity of the gamma cDNA for gamma mRNA sequences is strongly supported by the hybridization of this cDNA at ...
متن کاملThe beta-globin stage selector element factor is erythroid-specific promoter/enhancer binding protein NF-E4.
The analysis of transcriptional regulatory proteins is often hampered because such factors are present in cells in only sparing abundance. Although direct biochemical purification has been successfully applied to the analysis of many of these factors, such methods are labor intensive and expensive. We have developed an alternative strategy to identify and characterize such trans-acting factors ...
متن کاملGamma-globin gene promoter elements required for interaction with globin enhancers.
Normal expression of the human beta-globin domain genes is dependent on at least three types of regulatory elements located within the beta-globin domain: the locus control region (LCR), globin enhancer elements (3'beta and 3'Agamma), and the individual globin gene promoter and upstream regions. It has been postulated that regulation occurs through physical interactions between factors bound to...
متن کاملChicken /3-Globin Genes
We reported previously that DNA bend sites appear in the human /3-globin locus at an average distance of 680 bp. The relative locations of the sites were conserved among the five active /3-like globin genes and one pseudogene. Here, we mapped the sites in the /3-like globin genes from various species and examined their conservation. The locations of the bend sites in the bovine, rabbit and chic...
متن کاملSpecific repression of beta-globin promoter activity by nuclear ferritin.
Developmental hemoglobin switching involves sequential globin gene activations and repressions that are incompletely understood. Earlier observations, described herein, led us to hypothesize that nuclear ferritin is a repressor of the adult beta-globin gene in embryonic erythroid cells. Our data show that a ferritin-family protein in K562 cell nuclear extracts binds specifically to a highly con...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 1990
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.10.3.1116